Bookmark Sali - On problem with IRES vectors ?inability to transcribe the transcript following http://bookmarksali.win/story.php?title=on-problem-with-ires-vectors-inability-to-transcribe-the-transcript-following-11 On problem with IRES vectors ?inability to transcribe the transcript following the IRES sequence if the first MCS is empty. The new pIRES2-acGFP1acGFP1 vector was used as a control for the pIRES2Gal1-acGFP1 construct. Both vectors were sequenced through their multiple cloning sites to ensure no PCRinduced mutations were present.Transfection and stable clone generationParental, control, and galecti Fri, 09 Nov 2018 15:27:01 UTC en